mouse anti ap2 a adaptin 3b5 antibody  (Developmental Studies Hybridoma Bank)

Journal: Scientific Reports

Article Title: Francisella tularensis Uses Cholesterol and Clathrin-Based Endocytic Mechanisms to Invade Hepatocytes

doi: 10.1038/srep00192

Figure Lengend Snippet: (A, D) BNL CL.2 cells were infected with F. novicida for 8 h and immunolocalized with anti- F. novicida together with (A) anti-Eps15 (red) or (D) anti-AP-2 -adaptin (green) antibodies. Arrowheads indicate bacteria and protein co-localization. Scale bar = 5m. (B and E). Western blots show the overall protein levels of (B) Eps15 (145kDa) or (E) AP-2 -adaptin (50kDa) in 8 h F. novicida infected cells (untreated with RNAi) remained unchanged compared to uninfected control. Cells transiently transfected with siRNA (48 h) showed a significant reduction in the targeted Eps15 or AP-2 -adaptin proteins as compared to cells transfected with siRNA control pool (CP). (C and F) Reduced Eps15 and AP-2 -adaptin lead to a significant defect in F. novicida internalization 24 h post-infection. *P<0.05.

Article Snippet: A primary rabbit anti-Eps15 antibody (Santa Cruz Biotechnology, CA) was used at 1.0g/ml, a mouse anti-AP2 -adaptin (3B5) antibody (Trevor Williams lab, Developmental Studies Hybridoma Bank, IA) was used at 0.725g/ml, and a mouse anti-clathrin heavy chain antibody (BD Biosciences) was used at 0.25g/ml.

Techniques: Infection, Bacteria, Western Blot, Control, Transfection